Description

• One Shot™ Mach1™ T1R, (Cat. No. C862003), is the fastest-growing chemically competent strain currently available. Mach1™ T1R colonies are clearly visible within eight hours of plating your transformation. From an overnight colony, minipreps can be performed after four hours of growth.

• Genotype: F^- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rκ–, mκ +) ΔrecA1398 endA1 tonA
  • One Shot™ OmniMAX 2 T1R cells, (Cat. No. C854003), are perfect for use in all cloning applications, including Gateway™ technology. They offer the highest transformation efficiency of any chemically competent cell in the One Shot™ packaging format.
• Genotype: F' {proAB⁺ lacI^q lacZΔM15 Tn10(Tet^R) Δ(ccdAB)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80(lacZ)ΔM15 Δ(lacZYA-argF) U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD
  • One Shot™ Stbl3, (Cat. No. C7373-03), reduce the frequency of unwanted homologous recombination and so are perfect for cloning unstable DNA.
• Genotype: F^- mcrΒ mrr hsdcS20 (rΒ^-, mΒ^-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (Str^R) xyl-5 λ^- leu mtl-1
  • One Shot™ TOP10 cells, (Cat. No. C4040-03), are perfect for routine cloning and included in many TOPO™ cloning and expression kits.
• Genotype: F^- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (Str^R) endA1 nupG λ^-

Fast Growth: Yes
Blue-White Screening: Yes
Cloning Unstable DNA: Suitable for cloning unstable DNA
Library Construction: Yes
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Contains F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): Yes

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