内径x床高:10/300;柱体积:24ml;平均颗粒:11-15um;球蛋白分离范围(D):3000-700000;应用/特性:蛋白、肽、核苷酸及其它小分子的半制备和高性能分析纯化;耐反压:1.8MPa;最高流速:1.5ml/min。
|
Superose™ 10/300 GL Columns (Tricorn™)*
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Bed volume
|
24 ml
|
Sample loading capacity
|
5-10 mg, 240 μl
|
Recommended flow rate
|
|
Superose™ 6
|
0.3-0.5 ml/min
|
Superose™ 12
|
0.5-1.0 ml/min
|
Max. pressure
|
|
Superose™ 6
|
15 bar (217 psi, 1.5 MPa)
|
Superose™ 12
|
30 bar (435 psi, 3 MPa)
|
Particle size
|
|
Superose™ 6
|
13 ± 2 μm
|
Superose™ 12
|
10 ± 2 μm
|
Storage
|
20% ethanol
|
Storage temperature
|
4°C to 30°C
|
Chemical stability
|
Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
1% SDS, organic solvents,
0.5 M NaOH (for cleaning-in-place)
|
pH stability
|
3-12 (working and long term)
|
|
1-14 (short term)
|
Theoretical plates
|
|
Superose™ 6
|
> 30 000 m
-1
|
Superose™ 12
|
> 40 000 m
-1
|
Superose PC 3.2/30
|
Bed volume
|
2.4 ml
|
Max. loading capacity
|
200 μl
|
Max. flow rate
|
|
Superose 6
|
0.1 ml/min
|
Superose 12
|
0.1 ml/min
|
Max. pressure
|
|
Superose 6
|
12 Bar (1.2MPa / 175 psi)
|
Superose 12
|
24 Bar (2.4MPa / 350 psi)
|
Particle size
|
|
Superose 6
|
13 ± 2 μm
|
Superose 12
|
10 ± 2 μm
|
Chemical stability
|
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
|
pH stability
|
3-12 (working and long term) 1-14 (short term)
|
Theoretical plates
|
|
Superose 6
|
> 30 000 m-
1
|
Superose 12
|
> 40 000 m-
1
|
* Columns are not suitable for use with ÄKTAprime™ plus system.