LIF1005 | Leukemia Inhibitory Factor Protein, Recombinant human - 5 µg_蛋白质_生化试剂_常规试剂_高德真商城（goldengene.com）、品质保证、放心服务、轻松选购、仪器选型咨询、试剂耗材现货供应、比价我最优、比质我更好！

主要应用	品种	来源	纯度
CULT	Human	LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.	Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.

主要应用

品种

来源

纯度

CULT

Human

LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.

Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.

描述
产品目录编号	LIF1005
品牌系列	Chemicon®
同义词	LIF
商名	Chemicon
描述	Leukemia Inhibitory Factor Protein, Recombinant human - 5 µg
概述	Human LIF is a 19.7 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli expressed, recombinant human LIF is indistinguishable from native LIF in its biological activities in vitro. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells, than hLIF.
背景信息	Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

描述

产品目录编号

LIF1005

品牌系列

Chemicon®

同义词

LIF

商名

Chemicon

描述

Leukemia Inhibitory Factor Protein, Recombinant human - 5 µg

概述

Human LIF is a 19.7 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli expressed, recombinant human LIF is indistinguishable from native LIF in its biological activities in vitro. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells, than hLIF.

背景信息

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

产品信息
演示	Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

产品信息

演示

Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

应用
应用	Recombinant human Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.
主要应用	Cell Culture
应用说明	RECOMMENDED QC PROTOCOL M1 Bioassay 1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar. 2. Add 100 μL of sample or hLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes. 3. Add 100 μL of 5% FCS in isotonic saline to two control slides. 4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days. 5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated). Visit www.esgro-lif.com for additional information Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.

应用

Recombinant human Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.

主要应用

Cell Culture

应用说明

RECOMMENDED QC PROTOCOL

M1 Bioassay

1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.

2. Add 100 μL of sample or hLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.

3. Add 100 μL of 5% FCS in isotonic saline to two control slides.

4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.

5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).

Visit www.esgro-lif.com for additional information

Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.

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产品名称 :	LIF1005 \| Leukemia Inhibitory Factor Protein, Recombinant human - 5 µg
产品品牌 :	millipore/密理博

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LIF1005 | Leukemia Inhibitory Factor Protein, Recombinant human - 5 µg

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