LIF1010 | Leukemia Inhibitory Factor Protein, Recombinant human_蛋白质_生化试剂_常规试剂_高德真商城（goldengene.com）、品质保证、放心服务、轻松选购、仪器选型咨询、试剂耗材现货供应、比价我最优、比质我更好！

主要应用	品种	来源	纯度
CULT	Human	LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.	Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.

主要应用

品种

来源

纯度

CULT

Human

LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.

Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.

描述
产品目录编号	LIF1010
品牌系列	Chemicon®
同义词	LIF
商名	Chemicon
描述	Leukemia Inhibitory Factor Protein, Recombinant human
概述	Human LIF is a 19.7 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli expressed, recombinant human LIF is indistinguishable from native LIF in its biological activities in vitro. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells, than hLIF.
背景信息	Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

描述

产品目录编号

LIF1010

品牌系列

Chemicon®

同义词

LIF

商名

Chemicon

描述

Leukemia Inhibitory Factor Protein, Recombinant human

概述

Human LIF is a 19.7 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli expressed, recombinant human LIF is indistinguishable from native LIF in its biological activities in vitro. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells, than hLIF.

背景信息

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

产品信息
演示	Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

产品信息

演示

Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

应用
应用	Recombinant human Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.
主要应用	Cell Culture

应用

Recombinant human Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.

主要应用

Cell Culture

生物信息
浓缩	10 μg/mL
纯度	Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.
来源	LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.
品种	Human
特异活性	The activity of human LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of human LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(E8) units/mg, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

生物信息

浓缩

10 μg/mL

纯度

Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.

来源

LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.

品种

Human

特异活性

The activity of human LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of human LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(E8) units/mg, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

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产品名称 :	LIF1010 \| Leukemia Inhibitory Factor Protein, Recombinant human
产品品牌 :	millipore/密理博

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LIF1010 | Leukemia Inhibitory Factor Protein, Recombinant human

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