BlotCycler™ Mini全自动western blot系统

Succeed at Western Blotting! Introducing BlotCycler Mini…

Don’t you want to perform blocking and incubation protocols precisely every time you do a western blot? With BlotCycler™ Mini you can! BlotCycler Mini automates solution changes, blocking, incubation of primary and secondary antibodies, and washing steps.
Eliminate variables that occur by manually western blot – processing by hand has long been identified as the dominant source of variability in western blot results.*

Obtain consistent and accurate results over and over again, and increase productivity using automation. With rigorous precision, BlotCycler Mini delivers an affordable solution to busy labs demanding reproducible results.

How it helps you:

• Eliminates variations in fluid delivery, timing and washing that occur when protocols are performed manually
• Easily uses your standard protocols and reagents
• Remembers up to 100 programmed protocol steps, and saves up to 20 protocols in the internal memory

• Washes out the trays automatically after each test!

Description

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Succeed at western blotting…

You want clean western blot assay results – highly specific with a minimal background and a high signal. So, you make sure that every step in the process is performed precisely.

Proper labeling of the proteins with antibodies takes time. If blocking, washing and incubation are not done with the right materials in the exact amounts at just the right time, you’ve lost time because you don’t know if it’s good or bad until the experiment is over.

So, you perform every step like a robot, over and over – while you could be doing something more productive.

BlotCycler Mini performs every step precisely, accurately and – best of all – automatically. Blocking, antibody incubation and washing – everything! Just set it up and walk away.

Setting up BlotCycler Mini is easy. Simply add the blocking and washing buffers and primary and secondary antibodies. Program BlotCycler Mini to dispense them precisely when you want them. Set the timer to mix the solutions in a precise sequence. When the protocol is completed, BlotCycler automatically performs a wash cycle – and you’re ready for the next assay!

Blot-to-blot consistency. Time after time…

By eliminating variations in fluid delivery, timing and washing that occur when protocols are performed manually, BlotCycler Mini provides precise results. Using the same reagents, program as many as 100 protocol steps with up to 20 full protocols in the internal memory. Everything happens like clockwork, automatically. It even washes out the trays!

Not a micro-drop more or less

Never make another mistake when distributing solutions for your western blot protocol. BlotCycler Mini accurately dispenses blocking buffers, washing buffers, primary antibodies and secondary antibodies, the exact quantity you set. BlotCycler Mini selectively collects unused antibodies, too. It is like a trusted lab tech you can rely on in your lab.

Optimize, optimize, optimize…

Because it sets quickly and easily and it performs all the steps with rigid uniformity, BlotCycler Mini is the ideal for optimizing your western blot assays. Whether you’re optimizing the antibody dilution, determining the best concentration of blocking buffer, or testing for the best temperature for incubating antibodies, BlotCycler Mini performs the steps identically each time.

Western blot processing with the push of a button

Don’t you want to perform blocking and incubation protocols precisely every time you do a western blot? With BlotCycler™ Mini you can! BlotCycler Mini automates solution changes, blocking, incubation of primary and secondary antibodies, and washing steps.

The Proof:

Improved Specificity of Western Blot Following Processing at 4°C Using BlotCycler at 4°C Using BlotCycler

Two procedures involving incubating antibody were compared – manual and automated.

The manual procedure involved incubation of the primary antibody at 4°C while the rest of the procedure was performed at room temperature. The automated procedure involved performing all the processing steps 4°C using the using BlotCycler automated system.

Processing with BlotCycler™ show no nonspecific bands for a clearer result.

Manual procedure -please note the nonspecific bands seen on the blot.

Conclusion:

The data suggests that precise performance of blocking and washing steps in addition to the temperature at which these tasks were performed, eliminated nonspecific bands and generated high quality results.