LIF1005 | Leukemia Inhibitory Factor Protein, Recombinant human - 5 µg

  • 商品编号:
    LIF1005
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    ¥1760
  • 商品货号:
    LIF1005
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    ¥1760
  • 市场价格:
    ¥2112
  • 商品重量:
    1克
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  • 商品名称:LIF1005 | Leukemia Inhibitory Factor Protein, Recombinant human - 5 µg
  • 商品编号:LIF1005
  • 品牌:millipore/密理博
  • 上架时间:2015-04-01
  • 商品毛重:1克
  • 库存: 100 瓶

 

重要规格表

主要应用 品种 来源 纯度
CULT Human LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography. Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.

描述 
产品目录编号 LIF1005
品牌系列 Chemicon® 
同义词 LIF
商名
  • Chemicon
描述 Leukemia Inhibitory Factor Protein, Recombinant human - 5 µg
概述 Human LIF is a 19.7 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli expressed, recombinant human LIF is indistinguishable from native LIF in its biological activities in vitro. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells, than hLIF.
背景信息 Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

产品信息 
演示 Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

应用 
应用 Recombinant human Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.
主要应用
  • Cell Culture
应用说明 RECOMMENDED QC PROTOCOL 

M1 Bioassay 

1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar. 

2. Add 100 μL of sample or hLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes. 

3. Add 100 μL of 5% FCS in isotonic saline to two control slides. 

4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days. 

5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated). 

Visit www.esgro-lif.com for additional information 

Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.
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