LIF1010 | Leukemia Inhibitory Factor Protein, Recombinant human

  • 商品编号:
    LIF1010
  • 本店售价:
    ¥2620
  • 商品货号:
    LIF1010
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    ¥2620
  • 市场价格:
    ¥3144
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    1克
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  • 商品名称:LIF1010 | Leukemia Inhibitory Factor Protein, Recombinant human
  • 商品编号:LIF1010
  • 品牌:millipore/密理博
  • 上架时间:2015-04-01
  • 商品毛重:1克
  • 库存: 100 瓶

 

重要规格表

主要应用 品种 来源 纯度
CULT Human LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography. Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.

描述 
产品目录编号 LIF1010
品牌系列 Chemicon® 
同义词 LIF
商名
  • Chemicon
描述 Leukemia Inhibitory Factor Protein, Recombinant human
概述 Human LIF is a 19.7 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli expressed, recombinant human LIF is indistinguishable from native LIF in its biological activities in vitro. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells, than hLIF.
背景信息 Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

产品信息 
演示 Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

应用 
应用 Recombinant human Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.
主要应用
  • Cell Culture

生物信息 
浓缩 10 μg/mL
纯度 Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.
来源 LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.
品种 Human
特异活性 The activity of human LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of human LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(E8) units/mg, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
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