Description
Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).
Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection
Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:
| Fibroblast (3T3, COS-7) |
Myoblast (C2C12, L6 CRL-1458) |
| Hepatocyte (HepG2, HuH7) |
Erythroleukemic cell (K562) |
| Breast carcinoma (MCF7, Hs578T) |
Prostate carcinoma (LNCap) |
| Lung carcinoma (A549, NCI-H460) |
Osteosarcoma (U-2 OS, Saos-2) |
| Colon carcinoma (Caco2, SW480) |
Pancreatic carcinoma (PANC-1) |
| Skin melanoma (SK-MEL-28) |
|
Specifications
| Cell Type: |
Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells |
| Sample Type (Specific): |
Plasmid DNA, RNAi Plasmids (shRNA, miR), Synthetic siRNA |
| Classification: |
Animal Origin-Free |
| Transfection Technique: |
Lipid-Based Transfection |
| Serum Compatible: |
Yes |
| Product Line: |
Lipofectamine® |
| Product Size: |
1.5 mL |
Contents & storage
Contains one vial of Lipofectamine® 3000 Reagent (1.5 mL) and one vial of P3000TM Enhancer Reagent (1.5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. Store at 2–8°C. Do not freeze.
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